• Literature Based Project.• Word limit 10,000 words.• The word limit does not include the front title page, fly leaf page, acknowledgements, abstract, references and appendices.• Need enough of literature review and supported information, then critically evaluation of this information.• Supported by figures, tables, appendices, photos, and specific case examples.• 65 references (only textbooks, book chapters, and reviewed articles)• Referencing style is the Vancouver style.Research question:- What are the most factors that lead to false positive/ negative results of presumptive tests used to detect blood, saliva, and semen?Research objectives:- Investigate the factors that lead to false positive/ negative results of presumptive tests used to detect blood, saliva, and semen.The importance of this study is to:- Find the factors that lead to a false positive result for each presumptive tests.- Take these factors into consideration when using presumptive tests to detect blood, saliva, and semen.Chapter1. Introduction:• The importance of human body fluid in forensic investigation.• What is body fluid? How does it come to the crime scene?• The most common body fluid in crime scene• Why the focus will be on blood, saliva, and semen.• Presumptive tests: (Definition, Background, Purpose and value of using it in forensic investigation).• Resent presumptive tests commonly used to determine blood, saliva, and semen.• Why the result of the presumptive test is important.• False positive or negative result of presumptive tests: (what is it, how it comes, and the impact of forensic investigation)• This study aims to investigate the main factors that lead to false positive/ negative results in presumptive tests used to identify blood, saliva, and semen.Chapter2. Body fluid and presumptive test:2.1 blood:• Definition and component.• How it’s associated with crime.• Where it can be found in the crime scene.• Outcomes and value in forensic investigation.• Why it is important to identify blood in investigation.• The importance of presumptive tests for blood.• Blood presumptive tests:o Kastle-Meyer test.o Leucomalachite Green reagent.o Luminol test.(Brief background, component, mechanism, sensitivity, and positive and negative form)2.2 saliva:• Definition and component.• How it’s associated with crime.• Where it can be found in the crime scene.• Outcomes and value in forensic investigation.• Why it is important to identify saliva in investigation.• The importance of presumptive tests for saliva.• Phadebas test as saliva presumptive test:(Brief background, component, mechanism, sensitivity, and positive and negative form)2.3 Semen:• Definition and component.• How it’s associated with crime.• Where it can be found in the crime scene.• Outcomes and value in forensic investigation.• Why it is important to identify semen in forensic investigation.• The importance of presumptive tests for semen.• Acid Phosphatase (AP) Test as semen presumptive test:(Brief background, component, mechanism, sensitivity, and positive and negative form)Chapter 3. Factors lead to false positive/negative results with presumptive tests:- Factors lead to false positive/negative results with blood presumptive tests.- Factors lead to false positive/negative results with saliva presumptive tests.- Factors lead to false positive/negative results with semen presumptive tests.(Mean factors, mechanism of affecting, result form, consideration)Chapter 4. Conclusion: